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easysep mouse cd4+cd25+ regulatory t cell isolation kit ii  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc easysep mouse cd4+cd25+ regulatory t cell isolation kit ii
    Easysep Mouse Cd4+Cd25+ Regulatory T Cell Isolation Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+mouse+cd4+++cd25+++regulatory+t+cell+isolation+kit+ii/easysep+human+t+cell+isolation+kit/pmc12166341-100-16-25
    Average 90 stars, based on 1 article reviews
    easysep mouse cd4+cd25+ regulatory t cell isolation kit ii - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Cell Isolation:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Flow Cytometry:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Injection:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Control:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Staining:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Expressing:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Comparison:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Gene Expression:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Co-Culture Assay:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Derivative Assay:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Cell Culture:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    In Vitro:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Transduction:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Plasmid Preparation:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Activation Assay:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Marker:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Incubation:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Saline:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Adoptive Transfer Assay:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    MANN-WHITNEY:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Ex Vivo:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Labeling:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Differentiation Assay:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Cell Function Assay:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Functional Assay:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Methylation Sequencing:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Methylation:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Genome Wide:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Bioprocessing:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Recombinant:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Purification:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    RNA Sequencing:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Software:

    Article Title: Progranulin-dependent repair function of Regulatory T cells drive bone fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn–/– mice using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss
    Article Snippet: Using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL), CD4 + CD25 + cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Clonal hematopoiesis driven by mutated DNMT3A promotes inflammatory bone loss.
    Article Snippet: Using EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL), CD4+CD25+ cells were sorted to high purity (>95%) from Treg induction cultures.

    Article Title: Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single- cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Treg isolation, transduction, and expansion Mouse Tregs from splenocytes of C57Bl/6 or SJl mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Article Title: Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia
    Article Snippet: CD4+CD25+Foxp3+ T cells and CD4+CD25− T cells were isolated from splenocyte single‐cell suspensions by using an EasySep Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Stemcell Technologies, Vancouver, Canada), according to the manufacturers' protocols.

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing
    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis
    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).



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    ( A ) Representative images of colocalization of <t>CD4</t> (red) and FOXP3-GFP (green) in uninjured/injured bone tissue on day 0 (uninjury), day 3, day 7, day 14, and day 28 after operation. Scale bars: 100 μm (left); 10 μm (right). ( B ) Representative flow cytometry analysis of Tregs from callus tissue on day 0 (uninjury), day 3, day 7, day 14, and day 28 after operation. The numbers indicate the proportion of Tregs in the frame. ( C ) The proportions of Tregs among CD4 + TCRβ + cells, the numbers of Tregs, and the proportions of CD4 + cells at indicated time points in bone callus tissue (blue) and in SP (red). n = 4–5 per group. ( D ) Diagram illustration of Treg depletion by DT injection after bone-defect surgery. ( E ) Representative flow cytometry graphs of Treg proportions in control group (Cntrl) and Treg-depletion group. The numbers indicate the proportion of Tregs in the frame. ( F ) The proportions of Tregs among CD4 + TCRβ + cells. n = 5 per group. ( G ) Safranin O staining of injured bone tissues from the control group and Treg-depletion group. Scale bars: 200 μm. ( H ) The Safranin O images were analyzed using Image J, version 1.48. n = 4 per group. ( I ) H&E staining of injured bone tissues from the control group and Treg-depletion group. Scale bars: 200 μm. All data are represented as mean ± SEM. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.005; **** P ≤ 0.001, as determined by unpaired 2-tailed Student’s t test ( F and H ) or 1-way ANOVA with Bonferroni’s multiple-comparisons test ( C ).
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    Human MOG-CAR Tregs mediate target-antigen-specific immunosuppression and immunomodulation. ( A ) Suppression of human <t>CD4</t> polyclonally stimulated responder T cell (Tconv) proliferation by human MOG-CAR Tregs in co-culture. Human Tregs were pre-stimulated with culture media (None), anti-CD3/CD28 beads, or MOG-coated protein. The graph represents the mean of 5 donors. ( B ) Suppression of human CD8 polyclonally stimulated responder T cell proliferation by human MOG-CAR Tregs in co-culture. Human Tregs were pre-stimulated with culture media (None), anti-CD3/CD28 beads, or MOG-coated protein. The graph represents the mean of 4 donors. Two-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ( C ) MOG-CAR Tregs inhibited the maturation of monocyte-derived dendritic cells (DC). Autologous immature DC were treated with LPS to induce maturation (DC alone) or co-cultured with pre-stimulated MOG-CAR Tregs in the presence of LPS (CAR-Treg DC). After 3-days co-culture, DC phenotype was analyzed for surface expression of CD80, CD86, CD40, DC-SIGN and HLA-DR. One-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    Image Search Results


    ( A ) Representative images of colocalization of CD4 (red) and FOXP3-GFP (green) in uninjured/injured bone tissue on day 0 (uninjury), day 3, day 7, day 14, and day 28 after operation. Scale bars: 100 μm (left); 10 μm (right). ( B ) Representative flow cytometry analysis of Tregs from callus tissue on day 0 (uninjury), day 3, day 7, day 14, and day 28 after operation. The numbers indicate the proportion of Tregs in the frame. ( C ) The proportions of Tregs among CD4 + TCRβ + cells, the numbers of Tregs, and the proportions of CD4 + cells at indicated time points in bone callus tissue (blue) and in SP (red). n = 4–5 per group. ( D ) Diagram illustration of Treg depletion by DT injection after bone-defect surgery. ( E ) Representative flow cytometry graphs of Treg proportions in control group (Cntrl) and Treg-depletion group. The numbers indicate the proportion of Tregs in the frame. ( F ) The proportions of Tregs among CD4 + TCRβ + cells. n = 5 per group. ( G ) Safranin O staining of injured bone tissues from the control group and Treg-depletion group. Scale bars: 200 μm. ( H ) The Safranin O images were analyzed using Image J, version 1.48. n = 4 per group. ( I ) H&E staining of injured bone tissues from the control group and Treg-depletion group. Scale bars: 200 μm. All data are represented as mean ± SEM. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.005; **** P ≤ 0.001, as determined by unpaired 2-tailed Student’s t test ( F and H ) or 1-way ANOVA with Bonferroni’s multiple-comparisons test ( C ).

    Journal: The Journal of Clinical Investigation

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing

    doi: 10.1172/JCI180679

    Figure Lengend Snippet: ( A ) Representative images of colocalization of CD4 (red) and FOXP3-GFP (green) in uninjured/injured bone tissue on day 0 (uninjury), day 3, day 7, day 14, and day 28 after operation. Scale bars: 100 μm (left); 10 μm (right). ( B ) Representative flow cytometry analysis of Tregs from callus tissue on day 0 (uninjury), day 3, day 7, day 14, and day 28 after operation. The numbers indicate the proportion of Tregs in the frame. ( C ) The proportions of Tregs among CD4 + TCRβ + cells, the numbers of Tregs, and the proportions of CD4 + cells at indicated time points in bone callus tissue (blue) and in SP (red). n = 4–5 per group. ( D ) Diagram illustration of Treg depletion by DT injection after bone-defect surgery. ( E ) Representative flow cytometry graphs of Treg proportions in control group (Cntrl) and Treg-depletion group. The numbers indicate the proportion of Tregs in the frame. ( F ) The proportions of Tregs among CD4 + TCRβ + cells. n = 5 per group. ( G ) Safranin O staining of injured bone tissues from the control group and Treg-depletion group. Scale bars: 200 μm. ( H ) The Safranin O images were analyzed using Image J, version 1.48. n = 4 per group. ( I ) H&E staining of injured bone tissues from the control group and Treg-depletion group. Scale bars: 200 μm. All data are represented as mean ± SEM. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.005; **** P ≤ 0.001, as determined by unpaired 2-tailed Student’s t test ( F and H ) or 1-way ANOVA with Bonferroni’s multiple-comparisons test ( C ).

    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Techniques: Flow Cytometry, Injection, Control, Staining

    ( A ) Volcano plots represent differentially expressed genes between Treg-Cntrl cells and Treg-injury cells. n = 3 per group. ( B ) The experiments compare the percentage of CD44-, CTLA-, and KLRG1-positive Tregs from the uninjured bone (blue), Tregs from the wound/callus tissue at the injury site (red), Tregs from the SP of uninjured mice (orange), and injured mice (gray). n = 3–5 per group. ( C ) UMAP plot showing clusters and cluster annotations of BM T and NK cell (left panel). Stacked bar graph showing percentages of cells in each cluster among total NK and T lymphocytes (right panel). ( D ) UMAP plot showing clusters and cluster annotations of BM Tregs (left panel) and stacked bar graph showing percentages of cells in each cluster among total Tregs (right panel). ( E ) Violin plot showing the top markers for Treg1 and Treg2 subpopulations. ( F ) GO enrichment of the biological process categories in Treg2 subpopulation compared with Treg1 subpopulation. ( G ) Pseudotime analyses showing the differentiation of Treg1 subpopulation and Treg2 subpopulation from CD4 naive T cells. ( H ) Network diagram of the interaction between Treg1 (right panel) or Treg 2 (left panel) cluster and other cells in the BM. The size of the circle represents the number of interactions with all other types of cells, and the thickness of the line represents the interaction number of cells between the line. All data are shown as mean ± SEM. * P ≤ 0.05; *** P ≤ 0.005; **** P ≤ 0.001, as determined by 1-way ANOVA with Bonferroni’s multiple-comparisons test.

    Journal: The Journal of Clinical Investigation

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing

    doi: 10.1172/JCI180679

    Figure Lengend Snippet: ( A ) Volcano plots represent differentially expressed genes between Treg-Cntrl cells and Treg-injury cells. n = 3 per group. ( B ) The experiments compare the percentage of CD44-, CTLA-, and KLRG1-positive Tregs from the uninjured bone (blue), Tregs from the wound/callus tissue at the injury site (red), Tregs from the SP of uninjured mice (orange), and injured mice (gray). n = 3–5 per group. ( C ) UMAP plot showing clusters and cluster annotations of BM T and NK cell (left panel). Stacked bar graph showing percentages of cells in each cluster among total NK and T lymphocytes (right panel). ( D ) UMAP plot showing clusters and cluster annotations of BM Tregs (left panel) and stacked bar graph showing percentages of cells in each cluster among total Tregs (right panel). ( E ) Violin plot showing the top markers for Treg1 and Treg2 subpopulations. ( F ) GO enrichment of the biological process categories in Treg2 subpopulation compared with Treg1 subpopulation. ( G ) Pseudotime analyses showing the differentiation of Treg1 subpopulation and Treg2 subpopulation from CD4 naive T cells. ( H ) Network diagram of the interaction between Treg1 (right panel) or Treg 2 (left panel) cluster and other cells in the BM. The size of the circle represents the number of interactions with all other types of cells, and the thickness of the line represents the interaction number of cells between the line. All data are shown as mean ± SEM. * P ≤ 0.05; *** P ≤ 0.005; **** P ≤ 0.001, as determined by 1-way ANOVA with Bonferroni’s multiple-comparisons test.

    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Techniques:

    ( A ) Schematic diagram showing the CCL1 and CCR8 inhibitor treatment protocol. ( B ) Proportions of CCR8 + Tregs among Tregs and Tregs among CD4 + TCRβ + cells from uninjured group (sham, blue) and from injury site at control group (Cntrl, red), CCL1-treated group (CCL1, yellow), and CCR8 inhibitor–treated group (black) on 7 days after surgery. n = 3–5 per group. ( C ) The numbers of CCR8 + Tregs (left) and total Tregs (right) in bone callus tissue on P7. n = 5 per group. ( D ) The numbers of CCR8 + Tregs (left) and total Tregs (right) in adjacent lymph nodes on P7. n = 4 per group. ( E ) Frequencies of total SSC lineage cells, SSCs, BCSPs, and Thy1 + cells in total Lin – cells at the injury site on P7. n = 4–5 per group. ( F ) Safranin O staining (upper) and H&E staining (lower) of bone tissues at injury site in Cntrl, CCL1-treated group, and CCR8 inhibitor–treated group on P14 and P28, respectively. Scale bars: 200 μm. ( G ) The Safranin O images were analyzed using ImageJ, version 1.48. n = 5 per group. ( H ) Representative μCT images showing fracture healing. ( I ) Quantitative analysis of bone volume fraction (BV/TV) and bone volume of callus tissue. n = 5 per group. All data are presented as mean ± SEM. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.005; **** P ≤ 0.001, as determined by 1-way ANOVA with Bonferroni’s multiple-comparisons test.

    Journal: The Journal of Clinical Investigation

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing

    doi: 10.1172/JCI180679

    Figure Lengend Snippet: ( A ) Schematic diagram showing the CCL1 and CCR8 inhibitor treatment protocol. ( B ) Proportions of CCR8 + Tregs among Tregs and Tregs among CD4 + TCRβ + cells from uninjured group (sham, blue) and from injury site at control group (Cntrl, red), CCL1-treated group (CCL1, yellow), and CCR8 inhibitor–treated group (black) on 7 days after surgery. n = 3–5 per group. ( C ) The numbers of CCR8 + Tregs (left) and total Tregs (right) in bone callus tissue on P7. n = 5 per group. ( D ) The numbers of CCR8 + Tregs (left) and total Tregs (right) in adjacent lymph nodes on P7. n = 4 per group. ( E ) Frequencies of total SSC lineage cells, SSCs, BCSPs, and Thy1 + cells in total Lin – cells at the injury site on P7. n = 4–5 per group. ( F ) Safranin O staining (upper) and H&E staining (lower) of bone tissues at injury site in Cntrl, CCL1-treated group, and CCR8 inhibitor–treated group on P14 and P28, respectively. Scale bars: 200 μm. ( G ) The Safranin O images were analyzed using ImageJ, version 1.48. n = 5 per group. ( H ) Representative μCT images showing fracture healing. ( I ) Quantitative analysis of bone volume fraction (BV/TV) and bone volume of callus tissue. n = 5 per group. All data are presented as mean ± SEM. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.005; **** P ≤ 0.001, as determined by 1-way ANOVA with Bonferroni’s multiple-comparisons test.

    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Techniques: Control, Staining

    ( A ) Heatmap showing the expression of secreted factors in Tregs at injury site and Tregs in the control BM. n = 3 per group. ( B ) Volcano plot representation of a comparison of secreted gene-expression profiles between Treg1 subset and Treg2 subset. ( C ) Chromatin accessibility of the Grn locus in SP Tregs and BM Tregs in control and injury group. ( D ) Representative images of the expression of PGRN in CCR8 + Tregs and CCR8 – Tregs. ( E ) Quantification of the expression of PGRN in CCR8 + Tregs and CCR8 – Tregs. n = 4 per group. ( F ) Representative images showing the percentage of total Tregs, CCR8 + Tregs, and PGRN + CCR8 + Tregs in WT mice and Grn –/– mice. ( G ) Representative images of colonies of osteogenic (CFU-OB) and adipogenic (CFU-Adipo) assays showing the effect on the control with no Treg conditioned medium, with WT Treg conditioned medium, and with Grn –/– Treg conditioned medium. ( H ) Quantification of CFU-OB and CFU-Adipo colonies. n = 4–5 per group. ( I ) Statistical analysis of the proportions of Tregs in CD4 + TCRβ + cells, the proportion of CCR8 + Tregs among total Tregs, and the percentage of PGRN + CCR8 + Tregs in CCR8 + Tregs in WT mice and Grn –/– mice. n = 5 per group. ( J ) Diagram of the experimental protocol showing the transfer of WT/ Grn –/– Tregs into Treg-depletion mice after injury. ( K and L ) Safranin O staining of bone tissues on day 14 after injury operation (upper). H&E of bone tissues on day 28 after injury operation (lower). Scale bars: 200 μm. All data are shown as mean ± SEM. * P ≤ 0.05; *** P ≤ 0.005; **** P ≤ 0.001, as determined by 1-way ANOVA with Bonferroni’s multiple-comparisons test ( E and H ) or unpaired 2-tailed Student’s t test ( I ).

    Journal: The Journal of Clinical Investigation

    Article Title: Progranulin-dependent repair function of regulatory T cells drives bone-fracture healing

    doi: 10.1172/JCI180679

    Figure Lengend Snippet: ( A ) Heatmap showing the expression of secreted factors in Tregs at injury site and Tregs in the control BM. n = 3 per group. ( B ) Volcano plot representation of a comparison of secreted gene-expression profiles between Treg1 subset and Treg2 subset. ( C ) Chromatin accessibility of the Grn locus in SP Tregs and BM Tregs in control and injury group. ( D ) Representative images of the expression of PGRN in CCR8 + Tregs and CCR8 – Tregs. ( E ) Quantification of the expression of PGRN in CCR8 + Tregs and CCR8 – Tregs. n = 4 per group. ( F ) Representative images showing the percentage of total Tregs, CCR8 + Tregs, and PGRN + CCR8 + Tregs in WT mice and Grn –/– mice. ( G ) Representative images of colonies of osteogenic (CFU-OB) and adipogenic (CFU-Adipo) assays showing the effect on the control with no Treg conditioned medium, with WT Treg conditioned medium, and with Grn –/– Treg conditioned medium. ( H ) Quantification of CFU-OB and CFU-Adipo colonies. n = 4–5 per group. ( I ) Statistical analysis of the proportions of Tregs in CD4 + TCRβ + cells, the proportion of CCR8 + Tregs among total Tregs, and the percentage of PGRN + CCR8 + Tregs in CCR8 + Tregs in WT mice and Grn –/– mice. n = 5 per group. ( J ) Diagram of the experimental protocol showing the transfer of WT/ Grn –/– Tregs into Treg-depletion mice after injury. ( K and L ) Safranin O staining of bone tissues on day 14 after injury operation (upper). H&E of bone tissues on day 28 after injury operation (lower). Scale bars: 200 μm. All data are shown as mean ± SEM. * P ≤ 0.05; *** P ≤ 0.005; **** P ≤ 0.001, as determined by 1-way ANOVA with Bonferroni’s multiple-comparisons test ( E and H ) or unpaired 2-tailed Student’s t test ( I ).

    Article Snippet: For Treg transfer experiments, Tregs were freshly isolated from SPs of 8- to 10-week-old male WT and Grn –/– mice using EasySep Mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (catalog 18783, STEMCELL Technologies) as above.

    Techniques: Expressing, Control, Comparison, Gene Expression, Staining

    Human MOG-CAR Tregs mediate target-antigen-specific immunosuppression and immunomodulation. ( A ) Suppression of human CD4 polyclonally stimulated responder T cell (Tconv) proliferation by human MOG-CAR Tregs in co-culture. Human Tregs were pre-stimulated with culture media (None), anti-CD3/CD28 beads, or MOG-coated protein. The graph represents the mean of 5 donors. ( B ) Suppression of human CD8 polyclonally stimulated responder T cell proliferation by human MOG-CAR Tregs in co-culture. Human Tregs were pre-stimulated with culture media (None), anti-CD3/CD28 beads, or MOG-coated protein. The graph represents the mean of 4 donors. Two-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ( C ) MOG-CAR Tregs inhibited the maturation of monocyte-derived dendritic cells (DC). Autologous immature DC were treated with LPS to induce maturation (DC alone) or co-cultured with pre-stimulated MOG-CAR Tregs in the presence of LPS (CAR-Treg DC). After 3-days co-culture, DC phenotype was analyzed for surface expression of CD80, CD86, CD40, DC-SIGN and HLA-DR. One-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Journal: Journal of Neuroinflammation

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis

    doi: 10.1186/s12974-024-03262-w

    Figure Lengend Snippet: Human MOG-CAR Tregs mediate target-antigen-specific immunosuppression and immunomodulation. ( A ) Suppression of human CD4 polyclonally stimulated responder T cell (Tconv) proliferation by human MOG-CAR Tregs in co-culture. Human Tregs were pre-stimulated with culture media (None), anti-CD3/CD28 beads, or MOG-coated protein. The graph represents the mean of 5 donors. ( B ) Suppression of human CD8 polyclonally stimulated responder T cell proliferation by human MOG-CAR Tregs in co-culture. Human Tregs were pre-stimulated with culture media (None), anti-CD3/CD28 beads, or MOG-coated protein. The graph represents the mean of 4 donors. Two-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ( C ) MOG-CAR Tregs inhibited the maturation of monocyte-derived dendritic cells (DC). Autologous immature DC were treated with LPS to induce maturation (DC alone) or co-cultured with pre-stimulated MOG-CAR Tregs in the presence of LPS (CAR-Treg DC). After 3-days co-culture, DC phenotype was analyzed for surface expression of CD80, CD86, CD40, DC-SIGN and HLA-DR. One-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Techniques: Co-Culture Assay, Derivative Assay, Cell Culture, Expressing

    MOG-CAR Tregs engineered from MS patient blood are functionally active in vitro. ( A ) Percentage of naïve Tregs (CD25 hi CD127 lo CD45RA + ) among the CD4 cells of healthy donors and RRMS patients. ( B ) Fold expansion of healthy donors (grey) and RRMS patients (red) Tregs between day 0 to day 11 of the culture. ( C ) Percentage of transduction of the MOG-CAR with the lentivirus vector (at 1.10 7 UI/ml) in Tregs from healthy donors and RRMS patients. ( D ) Percentage of FOXP3 expression, FOXP3 TSDR hypomethylation and Helios expression on Tregs from healthy donors and RRMS patients. ( E ) Activation of MOG-CAR Tregs from healthy and RRMS patients analyzed using activation marker CD69 expression at cell surface of Tregs. Tregs were incubated 24 h with media, anti-CD3/CD28 beads or coated MOG (3 µg/ml). ( F ) Suppression of human CD4 polyclonally stimulated responder T cell (Tconv) proliferation by human MOG-CAR Tregs from healthy donors or RRMS patients in co-culture. Human Tregs were pre-stimulated with culture media (no activation), anti-CD3/CD28 beads, or MOG coated protein (3 µg/ml). Two-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01

    Journal: Journal of Neuroinflammation

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis

    doi: 10.1186/s12974-024-03262-w

    Figure Lengend Snippet: MOG-CAR Tregs engineered from MS patient blood are functionally active in vitro. ( A ) Percentage of naïve Tregs (CD25 hi CD127 lo CD45RA + ) among the CD4 cells of healthy donors and RRMS patients. ( B ) Fold expansion of healthy donors (grey) and RRMS patients (red) Tregs between day 0 to day 11 of the culture. ( C ) Percentage of transduction of the MOG-CAR with the lentivirus vector (at 1.10 7 UI/ml) in Tregs from healthy donors and RRMS patients. ( D ) Percentage of FOXP3 expression, FOXP3 TSDR hypomethylation and Helios expression on Tregs from healthy donors and RRMS patients. ( E ) Activation of MOG-CAR Tregs from healthy and RRMS patients analyzed using activation marker CD69 expression at cell surface of Tregs. Tregs were incubated 24 h with media, anti-CD3/CD28 beads or coated MOG (3 µg/ml). ( F ) Suppression of human CD4 polyclonally stimulated responder T cell (Tconv) proliferation by human MOG-CAR Tregs from healthy donors or RRMS patients in co-culture. Human Tregs were pre-stimulated with culture media (no activation), anti-CD3/CD28 beads, or MOG coated protein (3 µg/ml). Two-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01

    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Techniques: In Vitro, Transduction, Plasmid Preparation, Expressing, Activation Assay, Marker, Incubation, Co-Culture Assay

    MOG-CAR Tregs localize in the CNS of EAE mice. ( A ) and ( B ) are experiments performed in PLP induced EAE in SJL mice. Bioluminescence (photon/sec) of mice ( A ) and organs ( B ) from immunized or not immunized SJL mice. Mice were injected with MOG-CAR Tregs or with saline solution (No CAR Tregs). The graph shows the quantification of photons in the brain from day 9 to day 18. In ( B ) the graph shows the bioluminescence in the different organs at day 14. ( C ) was performed in another EAE model (adoptive transfer EAE). Number of mouse transduced Tregs (CD4 + FOXP3 + NGFR + ) in the CNS of EAE mice after treatment with MOG-CAR Tregs or CTRL CAR Tregs. Cell number under 20 were considered as a background. Mann Whitney test was performed. * p < 0.05

    Journal: Journal of Neuroinflammation

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis

    doi: 10.1186/s12974-024-03262-w

    Figure Lengend Snippet: MOG-CAR Tregs localize in the CNS of EAE mice. ( A ) and ( B ) are experiments performed in PLP induced EAE in SJL mice. Bioluminescence (photon/sec) of mice ( A ) and organs ( B ) from immunized or not immunized SJL mice. Mice were injected with MOG-CAR Tregs or with saline solution (No CAR Tregs). The graph shows the quantification of photons in the brain from day 9 to day 18. In ( B ) the graph shows the bioluminescence in the different organs at day 14. ( C ) was performed in another EAE model (adoptive transfer EAE). Number of mouse transduced Tregs (CD4 + FOXP3 + NGFR + ) in the CNS of EAE mice after treatment with MOG-CAR Tregs or CTRL CAR Tregs. Cell number under 20 were considered as a background. Mann Whitney test was performed. * p < 0.05

    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Techniques: Injection, Saline, Adoptive Transfer Assay, MANN-WHITNEY

    MOG-CAR Tregs delay the onset of EAE and reduce CNS inflammation. Monitoring of incidence ( A ) and clinical score ( B ) of passive EAE in C57Bl/6 mice. Mice were injected with pathogenic cells from donors’ mice and 24 h later MOG-CAR (pink), Ctrl-CAR Tregs (blue) or saline solution (grey) were injected i.v. Error bars represent mean ± SEM from 4 independent experiments including 33 mice/group. 2-way Anova Tukey’s multiple comparison was performed (Statistics MOG-CAR versus Saline: *<0.05, **<0.005, Statistics MOG-CAR versus CAR Ctrl: $<0.05, $$<0.005). ( C ) Percentage of IFN-γ positive CD4 cells after ex-vivo stimulation with MOG peptide. After mice sacrifice, cells recovered from the from CNS were incubated O/N with MOG peptide (10 µg/ml). 16 h later BFA was added to the media and intracellular staining was performed. Error bars represent mean ± SEM from 4 independent experiments including 33 mice/group. Stats: One-way ANOVA with Tukey’s multiple comparisons test. ( D ) The graph shows the mean score of demyelination for each spinal cord section. The mean score is (sum (number of spinal cord sections affected x severity)) / total number of spinal cord sections examined in each group. On the right: Representative transverse section of spinal cord from EAE mice stained with Luxol blue. Black arrows show demyelinated zone. ( E ) The graph represents the marked area after normalization in % of Iba-1 immunostainings obtained in spinal cord. Kruskal-Wallis test with multiple comparisons *<0.05, **<0.005. On the right: Representative transverse section of spinal cord from EAE mice stained with Iba-1 (brown staining)

    Journal: Journal of Neuroinflammation

    Article Title: MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis

    doi: 10.1186/s12974-024-03262-w

    Figure Lengend Snippet: MOG-CAR Tregs delay the onset of EAE and reduce CNS inflammation. Monitoring of incidence ( A ) and clinical score ( B ) of passive EAE in C57Bl/6 mice. Mice were injected with pathogenic cells from donors’ mice and 24 h later MOG-CAR (pink), Ctrl-CAR Tregs (blue) or saline solution (grey) were injected i.v. Error bars represent mean ± SEM from 4 independent experiments including 33 mice/group. 2-way Anova Tukey’s multiple comparison was performed (Statistics MOG-CAR versus Saline: *<0.05, **<0.005, Statistics MOG-CAR versus CAR Ctrl: $<0.05, $$<0.005). ( C ) Percentage of IFN-γ positive CD4 cells after ex-vivo stimulation with MOG peptide. After mice sacrifice, cells recovered from the from CNS were incubated O/N with MOG peptide (10 µg/ml). 16 h later BFA was added to the media and intracellular staining was performed. Error bars represent mean ± SEM from 4 independent experiments including 33 mice/group. Stats: One-way ANOVA with Tukey’s multiple comparisons test. ( D ) The graph shows the mean score of demyelination for each spinal cord section. The mean score is (sum (number of spinal cord sections affected x severity)) / total number of spinal cord sections examined in each group. On the right: Representative transverse section of spinal cord from EAE mice stained with Luxol blue. Black arrows show demyelinated zone. ( E ) The graph represents the marked area after normalization in % of Iba-1 immunostainings obtained in spinal cord. Kruskal-Wallis test with multiple comparisons *<0.05, **<0.005. On the right: Representative transverse section of spinal cord from EAE mice stained with Iba-1 (brown staining)

    Article Snippet: Mouse Tregs from splenocytes of C57Bl/6 or SJL mice were enriched using EasySep mouse CD4 + CD25 + Regulatory T Cell Isolation Kit II (STEMCELL Technologies).

    Techniques: Injection, Saline, Comparison, Ex Vivo, Incubation, Staining